The studies were undertaken with the objective to compare six staining techniques, nigrosineosin, eosin-nigrosin, try pan blue, Congo red-nigrosin, fast green-erythrocin and" fast green-eosin and select one of these staining techniques to measure live-dead sperm ratios of cock semen under the different length of time and temperature of holding semen.
The results obtained were summarized as follows:
Nigrosin-eosin, eosin-nigrosin and try pan blue staining techniques were estimated to be more, satisfactory methods and gave good results in live-dead spermatozoa differentiation in cock semen -
They showed the sharpest contrast between unstained sperm and the background and that try pan blue staining technique offered no critical smearing and drying procedure in which cells could be killed and result in a false high count.
Holding semen for 5 hours at 15°C as opposed to 30°C increased the incidence of unstained sperm but holding semen for 4 hours did not affect the incidence of stained sperm with increasing time of holding semen, there was a significant decline in percent of live sperm.
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